Supplemental materials to PhD Thesis "Validation of super-resolution 4Pi-microscopy, and its application to the study of intra-Golgi transport"
This dataset comprises the files contained on a CD-ROM which was attached to the thesis when it was submitted in 2009. It was uploaded to ORDO in 2025 for preservation purposes. For more information, please refer to the thesis “Validation of super-resolution 4Pi-microscopy, and its application to the study of intra-Golgi transport” via ORO.
Supplemental Video 3.1. The 3D overlap of the confocal-microscopy reconstruction made by the zero-crossing procedure, after 30% of initial thresholding (opaque), and the TEM recordings for the endosomal structures.
Supplemental Video 3.2. The 3D overlap of the confocal-microscopy reconstruction made by the zero-crossing procedure, after 30% of initial thresholding (transparent), and the TEM recordings for the endosomal structures.
Supplemental Video 3.3. The 3D overlap of the confocal-microscopy reconstruction made by the zero-crossing procedure, after 30% of initial thresholding (opaque), and the TEM recordings for the mitochondrial structures.
Supplemental Video 3.4. The 3D overlap of the confocal-microscopy reconstruction made by the zero-crossing procedure, after 30% of initial thresholding (transparent), and the TEM recordings for the mitochondrial structures.
Supplemental Video 3.5. The TEM 3D reconstruction of Golgi stack 1. Each colour represents a single Golgi cisternae or carrier.
Supplemental Video 3.6. The TEM 3D reconstruction of Golgi stack 2. Each colour represents a single Golgi cisternae or carrier.
Supplemental Video 3.7. The TEM 3D reconstruction of Golgi stack 3. Each colour represents a single Golgi cisternae or carrier.
Supplemental Video 3.8. The TEM 3D reconstruction of Golgi stack 4. Each colour represents a single Golgi cisternae or carrier.
Supplemental Video 3.9. The 3D overlap of the 4Pi-microscopy reconstruction made by threshold-intensity procedure (opaque) and the TEM recordings for Golgi stack 1. 164
Supplemental Video 3.10. The 3D overlap of the 4Pi-microscopy reconstruction made by the threshold-intensity procedure (transparent) and the TEM recordings for Golgi stack 1.
Supplemental Video 3.11. The 3D overlap of the 4Pi-microscopy reconstruction made by the zero-crossing procedure (opaque) and the TEM recordings for Golgi stack 1.
Supplemental Video 3.12. The 3D overlap of the 4Pi-microscopy reconstruction made by the zero-crossing procedure (transparent) and the TEM recordings for Golgi stack 1.
Supplemental Video 3.13. The 3D overlap of the 4Pi-microscopy reconstruction made by threshold-intensity procedure (opaque) and the TEM recordings for Golgi stack 2.
Supplemental Video 3.14. The 3D overlap of the 4Pi-microscopy reconstruction made by the threshold-intensity procedure (transparent) and the TEM recordings for Golgi stack 2.
Supplemental Video 3.15. The 3D overlap of the 4Pi-microscopy reconstruction made by the zero-crossing procedure (opaque) and the TEM recordings for Golgi stack 2.
Supplemental Video 3.16. The 3D overlap of the 4Pi-microscopy reconstruction made by the zero-crossing procedure (transparent) and the TEM recordings for Golgi stack 2.
Supplemental Video 3.17. The 3D overlap of the 4Pi-microscopy reconstruction made by threshold-intensity procedure (opaque) and the TEM recordings for Golgi stack 3.
Supplemental Video 3.18. The 3D overlap of the 4Pi-microscopy reconstruction made by the threshold-intensity procedure (transparent) and the TEM recordings for Golgi stack 3.
Supplemental Video 3.19. The 3D overlap of the 4Pi-microscopy reconstruction made by the zero-crossing procedure (opaque) and the TEM recordings for Golgi stack 3.
Supplemental Video 3.20. The 3D overlap of the 4Pi-microscopy reconstruction made by the zero-crossing procedure (transparent) and the TEM recordings for Golgi stack 3.
Supplemental Video 3.21. The 3D overlap of the 4Pi-microscopy reconstruction made by threshold-intensity procedure (opaque) and the TEM recordings for Golgi stack 4. 165
Supplemental Video 3.22. The 3D overlap of the 4Pi-microscopy reconstruction made by the threshold-intensity procedure (transparent) and the TEM recordings for Golgi stack 4.
Supplemental Video 3.23. The 3D overlap of the 4Pi-microscopy reconstruction made by the zero-crossing procedure (opaque) and the TEM recordings for Golgi stack 4.
Supplemental Video 3.24. The 3D overlap of the 4Pi-microscopy reconstruction made by the zero-crossing procedure (transparent) and the TEM recordings for Golgi stack 4.
Supplemental Video 3.25. The 4Pi-microscopy 3D reconstruction of the VSVG-pEGFP compartment in living COS7 cell made by the zero-crossing procedure (opaque). Yellow surfaces represents the co-localising regions.
Supplemental Video 3.26. The 4Pi-microscopy 3D reconstruction of the GaIT-venusYFP compartment in living COST cell made by the zero-crossing procedure (opaque). Yellow surfaces represents the co-localising regions.
Supplemental Video 3.27. The 4Pi-microscopy 3D reconstruction of the VSVG-pEGFP and GaIT?venusYFP combined compartments in living a COS7 cell made by the zero-crossing procedure (opaque).
Supplemental Video 3.28. The 4Pi-microscopy 3D reconstruction of the VSVG-pEGFP and GaIT?venusYFP combined compartments in living a COS7 cell made by the zero-crossing procedure (transparent). The co-localising volumes (opaque) are in yellow.
Supplemental Video 3.29. The 4Pi-microscopy 3D reconstruction of the VSVG-pEGFP and GaIT?venusYFP combined compartments in living a COS7 cell made by the zero-crossing procedure (transparent). The centres of gravity (opaque) are in green and red, respectively.
